Your goal is to convert biology protocols into python pseudocode. 
Output each pseudocode as a single-line string, wrapped in a Python code block. No need to include comments.

EXAMPLE
Here is an example of how to convert a protocol for recombinant protein expression and purification of codon-optimized Bst-LF polymerase into python pseudocode

example protocol:
1. Transform 100 ng of pET15b plasmid containing codon-optimized Bst-LF polymerase into E. coli C41 competent cells using either heat shock or electroporation.
2. Spread transformed cells in LB Agar plates supplemented with 0.1 mg/mL Amp. Grow plate overnight at 37 °C.
3. Select a single colony from the LB agar plate to prepare a preinoculum in 10mL LB media supplemented with 0.1 mg/mL Amp. Grow overnight at 250 rpm, 37 °C.
4. Use the full volume of the preinoculum to inoculate 1L of LB media supplemented with 0.1 mg/mL Amp. Grow at 200 rpm 18 °C until reaching an optical density at 600 nm (OD600) = 0.8.
5. Upon reaching OD600 = 0.8, add IPTG to a final concentration of 0.5 millimolar (mM) and incubate overnight at 200 rpm 18 °C.
6. Centrifuge the cell culture at 4000 x g, 4°C, for 30 minutes. Then, resuspend the cell pellet in 40 mL of Buffer A freshly supplemented with 1.0 millimolar (mM) PMSF and 0.2 mg/mL lysozyme.
7. Incubate the resuspended cells at 80 rpm, Room temperature (RT), for 30 minutes.
8. Sonicate on ice for 8 minutes using cycles of one second on ON and four seconds OFF at 40% amplitude
9. On an ultracentrifugation tube, incubate the unclarified lysate at 65 °C for 25 minutes to precipitate most of E. coli proteins, and then place on ice for 5 minutes. Centrifuge (20000 x g, 4°C, 30 minutes) and collect the supernatant. 
10. On a 5 mL HisTrap column pre-equilibrated with 10 column volumes (c.v.) (here, 50 mL) of Buffer A, load the supernant. Wash with 10-20 c.v. of Buffer B. Then, elute with 5 c.v. of Buffer C, collecting the eluted fractions every 1 mL in 1.5 ml tubes.
11. To pool the fractions containing the protein of interest, prepare a 96-well plate or 1.5 mL tubes with 40 µL of 5X Bradford reagent and 160 µL of distilled water. Add 10 µL of each protein fraction and compare against a blank reference sample corresponding to 10 µL of Buffer B. You can determine your protein-containing fractions either by absorbance at 595 nm on a plate reader or visually by comparing the blue coloration of each fraction against the blank reference. Pool your fractions.
12. To decrease the imidazole concentration, perform a buffer exchange step with an Amicon Ultra-15 concentrator. Centrifuge (3000 x g, 10°C, 10 minutes), discard the flowthrough, and add Buffer D to decrease the imidazole concentration. Repeat this step until the imidazole concentration reaches < 30 mM.
13. Recover the concentrated protein and determine its concentration using the Bradford assay. Then, supplement with 0.2 mM EDTA, 0.2 % volume Triton X-100, 2 mM DTT and add glycerol up to 50 % volume to reach Storage Conditions.


example python pseudocode:
transformed_cells = transform_cells(input="E. coli C41", plasmid="pET15b-Bst-LF", method="heat shock")
transformed_cells = grow_cells_on_agar(input=transformed_cells, amp_conc="0.1 mg/mL", temperature=37, time="overnight")
preinoculum = prepare_preinoculum(input=transformed_cells, colony="single colony", media_vol="10 mL LB", amp_conc="0.1 mg/mL", rpm=250, temperature=37)
inoculated_cells = inoculate(input=preinoculum, preinoculum_vol="full volume", media_vol="1 L LB", amp_conc="0.1 mg/mL", rpm=200, temperature=18, time="until OD600 = 0.8")
induced_cells = induce_protein_expression(input=inoculated_cells, iptg_conc="0.5 mM", rpm=200, temperature=18, time="overnight")
harvested_cells = harvest_cells(input=induced_cells, culture="1 L culture", centrifuge_settings="4000 x g, 4°C, 30 minutes")
resuspended_cells = resuspend_cells(input=harvested_cells, buffer_vol="40 mL", buffer_composition="Buffer A + 1.0 mM PMSF + 0.2 mg/mL lysozyme")
incubated_cells = incubate_cells(input=resuspended_cells, incubation_params="80 rpm, RT, 30 minutes")
lysed_cells = lyse_cells(input=incubated_cells, sonication_params="8 minutes, 40% amplitude")
incubated_lysate = incubate_cells(input=lysed_cells, incubation_params="65 °C, 25 minutes")
chilled_lysate = place_on_ice(input=incubated_lysate, time="5 minutes")
centrifuged_lysate = centrifuge_cells(input=chilled_lysate, centrifuge_settings="20000 x g, 4°C, 30 minutes")
protein_in_lysate = purify_protein(input=centrifuged_lysate, purification_params="HisTrap column, 10 c.v. Buffer A, 10-20 c.v. Buffer B, 5 c.v. Buffer C")
protein_containing_fractions = identify_protein_fractions(input=protein_in_lysate, bradford_params="40 µL 5X Bradford reagent + 160 µL distilled water + 10 µL protein fraction")
protein_solution = buffer_exchange(input=protein_containing_fractions, buffer_composition="Buffer D", centrifuge_settings="3000 x g, 10°C, 10 minutes", repetitions="until imidazole concentration < 30 mM")
concentrated_protein = finalize_protein_concentration(input=protein_solution, storage_composition="0.2 mM EDTA + 0.2 % volume Triton X-100 + 2 mM DTT + 50 % volume glycerol")


YOUR TASK:
Here is a molecular biology protocol entitled '{title}' The protocol steps are as follows:

{protocol}

Please convert this protocol into python pseudocode.

python pseudocode: